Banca de DEFESA: JULIA DE MELO JUREMA GUIMARÃES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIA DE MELO JUREMA GUIMARÃES
DATE: 07/12/2023
TIME: 09:00
LOCAL: Sala 07, Faculdade de Farmácia, campus Ondina
TITLE:

Effect of pH on survival and biofilm formation by Leptospira

KEY WORDS:

spirochetes, leptospirosis, pH, stress

PAGES: 42
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Leptospires are Gram negative bacteria with a wide physical-chemical agents sensibility profile, previous works revealed that Leptospira may survive for months in water and soil. Therefore, leptospires remain as a relevant human and veterinary health issue. Leptopsira form biofilms in vitro, in vivo and on the environment. However, there is no literature to our knowledge describing if continuous exposition to unfavorable temperatures and pHs may or not alter biofilm formation in Leptospira. The aim of this work is to evaluate pH and temperature stress action in biofilm formation and cell survival. Leptospiral biofilm biomass cultivated at 30°C with pHs 5.0; 6.5; 7.5 e 8.5 was quantified using crystal violet stain method. We generated growth curves of planktonic Leptospira at 30 °C with pH 5.0; 7.5 and 8.5. Preliminary results show that pH 5.0 and elevate temperatures cultivation conditions inhibit biofilm formation in Leptospira. The present results alongside our future results may enlighten if physycal-chemical stress exposition interfere in Leptospira biofilm formation

COMMITTEE MEMBERS:
Presidente - 1879071 - PAULA CARVALHAL LAGE VON BUETTNER RISTOW
Interno - ***.173.536-** - RODRIGO DIAS DE OLIVEIRA CARVALHO - UFMG
Externa à Instituição - LUCIANA DOS SANTOS MEDEIROS - UFF